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Determination of the cell viability and intracellular actin distribution in HeLa cells using fluorescence microscopy

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Write a report on determination of the cell viability and intracellular actin distribution in HeLa cells
using fluorescence microscopy.

The Introduction should include a review of or reference to the most recent relevant
literature. It should be fully referenced. In addition it should clearly state why you care about
this experiment
i.e. what is its significance. It should clearly state the question(s) you are
asking. Consequently, you should also state how you are going to answer the questions you
are posing. Finally the last sub-section of your introduction should be called
“Aims of this
laboratory experiment”.
The Materials and Methods Section should document the materials and apparatus you
used and document the methods you used. Photos of apparatus are not necessary. In brief,
explain what you did in such a way that it could be repeated by an intelligent layperson. There
is no value here in reiterating the methods section of the lab protocol. Please only document
instances where you have deviated from (or adapted) the schedule provided.
The Results Section is mainly text describing what you found out. Graphs and tables etc
should be used to support the text (not the other way round). You should also use the results
section as an opportunity to point out whatever it is that you want to draw to the attention of
the reader towards.
The Discussion Section is where you explain and discuss your findings (results) in the
context of the current literature base
i.e. your results should be compared with other
investigators’ work, and any differences / similarities noted. Perhaps your work confirms other

peoples’ findings – or it may not be the same. Give reasons or suggestions as to why this
might be so. Use the discussion to explain your results. The discussion is an important part of
your project, and needs to be critical and evaluative – it must analyze and explain your
findings in comparison with those of others. Which are more reliable, and why? Did you
confirm what others have found (that’s fine), or did you find something different (why was this
the case?). This should be more than several pages. One paragraph is not sufficient! You
may also want to include a future work section
. This should be a fairly short, indicating (based
on your own work) what needs to be done in the future to take the work further. What
questions are raided by this work? How would you answer them?
The Conclusion Section is where you explain the outcomes of your work ‘I found X and this
means Y’. The conclusion is not supposed to be a list of bullet points: you need to explain
your conclusions, not list them. Say what have you found out in a concise fashion.
The References Section should be a list, in alphabetical order. You should use the Harvard
system of referencing. Do not use URLs or cite wikis (under any circumstances!). Use books,
research papers and review articles. Anything with a doi is OK). References such as
“PUBMED” or “Science Direct” are not specific and will be ignored. You need to cite the
paper!

Introduction

Cell viability intracellular actin distribution examination in culture stands as a significant method of assessing in vitro medicine or environmental impacts in cytotoxicity analyzes or for tracking cell multiplication and vigor in culture. Assays conducted using Fluorescence for cell feasibility measurement are among the commonest cellular based assays conducted nowadays. Characteristically, viability of cells is evaluated through differentially marking living and dead cells, then coming up with the proportion of the cells that are live using fluorescent microscopy. The dyes for the living cells usually entail an integral metabolizing and processing cell while the dyes for the dead cells label cells where the plasma membrane integrity has been distorted (Mahbub et al., 2017, p.15792).

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